FTIR Spectroscopic Study Of Inhibition of Chloroxylenol-Based Disinfectant Against Salmonella enterica serovar Thyphimurium Biofilm
نویسندگان
چکیده
The present work was performed to determine the impacts of commercial disinfectants against biomass, viability, and biochemical composition Salmonella enterica serovar Thyphimurium ATCC14028 biofilm. biofilm grown in microplates exposed namely sodium hypochlorite, benzalkonium chloride, chloroxylenol, dodecyl-benzene sulfonate-based disinfectants. Biofilm were determined using crystal violet assay, resazurin assay Fourier transform infrared (FTIR) spectroscopy respectively. Results demonstrated that, among four disinfectants, chloroxylenol-based disinfectant showed highest inhibition S. It remarkably hindered biomass viability at all tested concentrations (0.78%-25%). Half-maximal inhibitory concentration (BIC50) (5.06%) found be lowest Meanwhile, treated with exhibited changes FTIR spectral peaks associated lipid (1460 cm-1), protein (630 cm-1, 702 1550 cm-1 & 1650 nucleic acid (1080 1229 cm-1). findings study suggest that is mediated by structural
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ژورنال
عنوان ژورنال: Malaysian applied biology
سال: 2023
ISSN: ['0126-8643', '2462-151X']
DOI: https://doi.org/10.55230/mabjournal.v52i2.2614